Zeiss AxioCam MRc5 Instrukcja Użytkownika Pobierz pdf (Strona 5)

actual colors seen in the microscope ocular / eye-piece, one can alternatively try the 3200K

standard setting (as mentioned below).

For brightfield / DIC imaging, do an interactive white balance by clicking on the "Interactive..."

box, and then use the cursor and left click mouse on a "background white region." The red-

green-blue (RGB) peaks in the image Histogram should ideally overlap (color balance), and also

be in the mid-region of the intensity grayscale (0 — 4095). As mentioned above, changes to the

image color can be done manually by clicking on the arrows next to the various color spots.

Under General in the menu of the AxiocamMRc5 box (below), the digital gain can be selected;

typically, an index value of 0 (gain factor of 1) is used (default). Image Orientation is Original

(default).

The background correction (Shading Correction) removes a digital background image which

may be noticed on the live image from the camera. [This kind of "background visual material" is

not due to material on your slide, but is in the camera / imaging system itself, and is a

"constant."] If you do not see or notice much "artifact" or annoying "material" in the

background, then you probably do not "need" to do a shading correction. However, if there is a

need or desire to do so: after doing the focus and brightness adjustments on your sample,

move to a region that does not have cells and is also very "clean and clear- (moving to a region

off the slide can work also). Click on the Shading Correction box - this will "clear or blank" the

background, and ideally nothing should then be seen in the image box. The Enable box should

then have a check. When you move back to the regions of your specimens (cells, tissue or

whatever), there should be

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Zeiss AxioCam MRc5 Instrukcja Użytkownika Strona 5