Zeiss Axioplan 2 imaging and Axiophot 2 Podręcznik Użytkownika Pobierz pdf (Strona 11)

Figure 8. Buttons for changing filter position.

Make sure that you are using a filter that matches the spectral properties of the fluorochromes in

your sample. The web page of the instrument contains the links to the spectral information of each

filter.

The triple filter in microscope 2 is not intended for imaging. Instead, for multilabel imaging, use the

multidimensional acquisition with individual filters for each label.

5 Objectives

5.1 List of objectives available

There are six objectives in the microscopes:

Microscope 1

Mag.

Type

Working

distance

(mm)

Numerical

Aperture

(N.A.)

Immersion

medium

Phase ring

position

DIC

position

10x

Plan-Neofluar

5.6

0.3

air

Ph1

20x

EC Plan-Neofluar

2.0

0.5

air

Ph2

DIC II

40x

EC Plan-Neofluar

0.71

0.75

air

Ph2

DIC II

63x

Plan-Neofluar

0.1

1.25

oil

DIC III

63x

Plan-Neofluar

0.1

1.25

oil

Ph3

100x

Plan-Neofluar

0.2

1.3

oil

Ph3

DIC III

1 2 ... 6 7 8 9 10 11 12 13 14 15 16 ... 27 28

Axiovision software 1
1 General 4
2 Turning on the equipment 5
2.4 Axiocam HRc camera 7
3 Light paths 8
4 Filters 9
5 Objectives 11
5.2 Oil objectives 12
6 Placing the sample 13
7 Focusing 14
8.1 Settings 15
8.2 Bright-field imaging 16
8.3 Phase contrast imaging 16
8.4 DIC imaging 16
9 Adjusting the oculars 18
10 Acquiring images 18
10.4 Exposure time 19
10.5 White balance 20
10.6 Image resolution 21
10.7 Display properties 21
10.9 Z-stack imaging 23
11 Post processing 24
13 Exporting images 25
15 Troubleshooting 27
15.3 Microscope 28

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Zeiss Axioplan 2 imaging and Axiophot 2 Podręcznik Użytkownika Strona 11

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