Zeiss Axioplan Universal microscope Instrukcje Operacyjne Strona 10
- Strona / 17
- Spis treści
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Oceniono. / 5. Na podstawie oceny klientów
B 40 - 017 e / 12.96
8
4 Instructions for use
4.2 Preparation of universal rotary
stage
4.2.1 Positioning the sample
Select the suitable segment pair (Fig. 1/
6
and
7
), The refractive indices of the sample and the
segment pair should be almost identical. For
quartz or feldspa crystals, for example, the
segment pair featuring the refractive index
n
D
= 1,556 should be selected, for pyroxen or
amphibol crystals the one with the refractive
index n
D
= 1,648.
Insert the larger, lower segment (Fig. 1/
7
) into
the annular bearing (Fig. 3/
25
) to fit the groove.
Apply immersion fluid, e.g. glycerin, to the flat
segment surface.
Position the sample and put one drop of im-
mersion fluid on it.
Center the upper segment (Fig. 1/
6
) parallel to
the sample so that the screws (Fig. 3/
22
) touch
the threaded bores in question. Lightly tighten
the screws so that the immersion fluid is evenly
distributed and shows no bubbles.
Align the objective on the nosepiece with the
rotary axis A
5
according to the microscope
manual. (For the
Axioplan Pol
,
Axioplan 2 Pol
,
Axiophot Pol
and
Axiophot 2 Pol
microscopes,
check the center-ing of the microscope stage
and re-center it, if necessary.)
4.2.2 Imaging the sample
Sharply image the sample and use the con-
denser to illuminate it (as specified in the mi-
croscope manual). Sharply image the nar-
rowed luminous field diaphragm together with
the sample, center and open it until the field of
view is visible.
4.2.3 Centering the universal rotary stage to the
microscope axis
The universal rotary stage of the
Axiolab Pol
has already been centered by the manufac-
turer.
With the polarization microscopes
Axioplan Pol
,
Axioplan 2 Pol
,
Axiophot Pol
and
Axiophot 2 Pol
the universal rotary stage must be centered
relative to the rotary axis of the microscope
stage.
Arrest the microscope stage (Fig. 4/
8
). Rotate
the sample through axis A
1
. Use the adjusting
screws (Fig. 4/
9
and
11
) and pin key (Fig. 1/
9
) to
align the rotary axis of the universal rotary stage
relative to the rotary axis of the microscope
stage in accordance with the crosslines visible
in the eyepieces, tighten the clamping screws
(Fig. 4/
6
und
12
) with the ball-head screwdriver
(Fig. 1/
12
) (This secures the centering).
4.2.4 Vertical adjustment of sample
Loosen the clamps of axis A
4
(Fig. 3/
26
) and
observe the sample as it is tilting. If the sample
moves in the direction of rotation, it is located
too high. If it moves opposite to the direction of
rotation, it is located too low.
If the specimen remains unchanged when it is
tilted about axis A
4
, it is at the correct height. In
this case the intersections of the universal rotary
stage axes, the center point of the sphere and
the focus are located in this point, which is in-
tersected by the surface of the sample.
If the sample is too high, use the pins (Fig. 3/
6
)
to turn the threaded ring
counter-clockwise
as
seen from below. When the correct height has
been reached, lightly tighten the screws
(Fig. 3/
22
).
If the sample is too low, loosen screws
(Fig. 3/
22
) and turn the threaded ring (Fig. 3/
6
)
clockwise
as seen from below. When the cor-
rect height has been reached, lightly tighten
the screw (Fig. 3/
22
), if necessary.
4.2.5 Aligning the A
2
and A
4
axes to the eyepiece
crosslines
Tilt the universal rotary stage about the A
4
and
A
2
axes while observing the direction in which a
dust particle below or above the object plane
is moving.
When the stage is tilted about the A
4
axis, the
particle must move parallel to the vertical line
of the eyepiece crosslines. It it fails to do so,
disengage the stage click stop mechanism
and perform the necessary alignment by rotat-
ing the stage through the A
5
axis..
Activate the stage click stop mechanism.
When the stage is tilted about the A
2
axis, the
particle must move parallel to the horizontal
(left-right), when tilted about the A
4
axis, par-
allel to the vertical eyepiece crossline (top-
bottom). This is the standard setting.
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