Zeiss Axiovert 200 Instrukcja Użytkownika Pobierz pdf

27 Jan 2015,<qsg-wf1> p. 1/8

UICK

TART

UIDE

IDEFIELD

EISS

XIOVERT

200

OLOCITY

(SAFB

409)

Startup procedure ................................................................................................................ 2

Observing through the eyepiece .......................................................................................... 2

Brightfield: ................................................................................................................................... 3

Fluorescence: ............................................................................................................................. 3

Adjusting brightfield Köhler illumination ............................................................................... 3

Acquiring the image ............................................................................................................. 4

Multidimensional setup ........................................................................................................ 5

Multiple channels ........................................................................................................................ 5

Z stacks ...................................................................................................................................... 5

Time series ................................................................................................................................. 6

Multi-position ............................................................................................................................... 6

Manual positioning .................................................................................................................................... 6

Automated positioning .............................................................................................................................. 6

Stitching ...................................................................................................................................... 6

Start Capture ............................................................................................................................... 7

Shutdown procedure ............................................................................................................ 7

Other controls ...................................................................................................................... 8

Auto Exposure ............................................................................................................................ 8

Auto Contrast .............................................................................................................................. 8

Keypad controls: ......................................................................................................................... 8

File formats .......................................................................................................................... 7

Facility for Imaging

by Light Microscopy

Observing Life As It Happens

Imperial College London

1 2 3 4 5 6 7 8

Podsumowanie treści

Strona 1 - Imperial College London

27 Jan 2015,<qsg-wf1> p. 1/8 QUICKSTART GUIDE: WIDEFIELD 1 ZEISS AXIOVERT 200 / VOLOCITY (SAFB 409) Startup procedure ...

Strona 2 - Startup procedure

27 Jan 2015,<qsg-wf1> p. 2/8 Startup procedure Turn on (in this order): • if required: heater unit and heater, 4 hours before live imaging to

Strona 3 - Fluorescence:

27 Jan 2015,<qsg-wf1> p. 3/8 The shutters can be controlled either in the software or on the microscope Brightfield: - Select channel 1 in Vo

Strona 4 - Acquiring the image

27 Jan 2015,<qsg-wf1> p. 4/8 Acquiring the image • On the microscope stand - change the light path to BASEPORT • In Volocity - select channel

Strona 5 - Multidimensional setup

27 Jan 2015,<qsg-wf1> p. 5/8 Multidimensional setup Multiple channels • open "Acquisition Setup" dialogue • tick "Change cha

Strona 6 - Automated positioning

27 Jan 2015,<qsg-wf1> p. 6/8 Time series • in "Acquisition Setup" select the “Time” tab • set intervals • set duration Multi-p

Strona 7 - File formats

27 Jan 2015,<qsg-wf1> p. 7/8 Start Capture To start(/stop) recording - click on the red button icon on the video toolbar you start your record

Strona 8 - Other controls

27 Jan 2015,<qsg-wf1> p. 8/8 Other controls Auto Exposure • sets max pixels to 80% (not 100%) (can also be applied to a selected area) Auto C

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