Zeiss LSM 510 META Dokumentacja Strona 252

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OPERATION
Annex LSM 510
4-194 B 40-051 e 07/98
Amplifier Gain
The diode signal is set to the right range (graylevel between 50 and 200 - 8bit image / 750 and
3500 - 12 bit image) with the help of gray filters and amplifier gain. The use of a lower filter density
should be priorized against the use of a high gain value. The value of the amplifier gain of both channels
(PMT and diode) should be set to one if possible. Because of a, parallel to the gain factor, increasing
amplifier noise, a gain value of more than 2 should be avoided. The most important thing is, that no
pixels are below the zero level and beyond the maximum range respectively.
Fig. 4-180
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