
INTRODUCTION TO LASER SCANNING MICROSCOPY
Functions LSM 510
5-48 B 40-051 e 07/98
If Blue/Red is selected, the voxels with grey values within the interval Low, High remain unchanged.
Voxels with grey values less than Low are highlighted in blue; those with grey values higher than High
are highlighted in red.
If the Invert option is selected, the grey values outside the defined interval will be segmented.
If the option Binary is selected, then all grey values in the range from Low to High will be set to white
(grey value 255 (4095)) in the Output image sequence, while all others will be set to black (grey value
0). If the option is not selected, the grey values within the selected interval remain unchanged, while
those outside the range will be set to black.
Parameters:
Input Input image sequence
Output Resulting image sequence
Colour Green - Selected interval is displayed in green
Blue/Red - Grey values below the selected interval are displayed in blue, grey
values above in red
Binary 0 - Selected voxels retain the original grey value
1 - Selected voxels are set to grey value 255 (4095), the rest to grey value 0
Invert 0 - Grey values inside the selected interval are segmented
1 - Grey values outside the selected interval are segmented
L Low grey value threshold
C Center of threshold interval
H High grey value threshold
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