Zeiss Axiovert 135M Dokumentacja Pobierz pdf (Strona 43)

Axiovert 25, Axiovert 25 C, Axiovert 25 CFL

B 40Ć012 e 08/99 2Ć9

2.3 Use of LD objectives

When inverted microscopes are used for analyses, the bottom thickness of the vessels used differs

considerably from the standard cover slip thickness of 0.17 mm.

Normally, such distances are easily bridged by the working distances of lowĆpower objectives:

- CP Achromat 5x/0.12 FWD (in air) 10.8 mm or 

- CP Achromat 10x/0.25 FWD (in air) 5.1 mm.

Already in the middle magnification range, however, these working distances are reduced to values

around or below 1 mm. Such objectives can then no longer be used for thicker bottoms.

This shortcoming is remedied by special LD

(Long Distance) objectives; these objectives

feature a relatively long working distance, but

also the normal 45 mm parfocalization length of

all other objectives.

For adaptation to the bottom thickness in use,

fit the corresponding cover glass cap (2Ć12ā/ā1)

onto the objective (see table below for assignĆ

ments).

Table of free working distances on the Axiovert 25

Objective name Order No. FWD

without cover glass cap

for bottom thickness

FWD

with cover glass cap

for bottom thickness

D=0.17

[mm]

D=1

[mm]

D=1.2 - 1.8

[mm]

0 Ć 0.6

(444931)

[mm]

0.6 Ć 1.2

(444930)

[mm]

CPĆAchromat 5xā/ā0.12 Ph0 440921 10.7 10.3 10.2 ... 9.9* - -

CPĆAchromat 10xā/ā0.25 Ph1 440931 5.0 4.6 4.5 ... 4.2* - -

CPĆAchromat 10xā/ā0.25 Ph1/Var1 440938 5.0 4.6 4.5 ... 4.2* - -

LDĆAchrostigmat 20xā/ā0.30 Ph1 440147 2.2 1.8 1.7 ... 1.4* - -

LDĆAchrostigmat 20xā/ā0.30 Ph1/Var1 440148 2.2 1.8 1.7 ... 1.4* - -

LDĆAchrostigmat 32xā/ā0.40 Ph1 440149 2.5 2.1 2.0 ... 1.7* - -

LDĆAchrostigmat 32xā/ā0.40 Ph1/Var1 440146 2.5 2.1 2.0 ... 1.7* - -

LDĆAchrostigmat 40xā/ā0.55 Ph2 440155 - - 1.8 ... 1.5* 1.6 ... 1.3* 1.6 ... 1.3*

* depending on bottom thickness

The working distances of PhVar objectives correspond to those of the Ph objectives.

Figure 2Ć12   Working with LD objectives

1 2 ... 38 39 40 41 42 43 44 45 46 47 48 ... 75 76

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