Zeiss Axiovert 135M Dokumentacja Pobierz pdf (Strona 49)

Axiovert 25, Axiovert 25 C, Axiovert 25 CFL

B 40Ć012 e 08/99 2Ć15

NOTE • Shifting the VAREL illumination to outside the pupil corresponds to oneĆsided

darkfield illumination.

• Shifting the VAREL illumination between the Ph and VAREL rings of the objective

corresponds to inclined brightfield illumination.

• The middle engaging position of the Phā/āHā/āVar slider permits brightfield

illumination.

• The left engaging position of the Phā/āHā/āVar slider permits the fast change from

VAREL to Ph contrast.

Figure 2Ć20 Pupil images in VAREL contrast

Objective

VAREL contrast

Inclined brightfield

illumination

OneĆsided darkfield

illumination

Ph ring VAREL ring VAREL diaphragm

Preparing the Var/H/Var slider for phase contrast

• Remove the attenuation filter (2Ć21ā/ā3) from

the center position of the slider (remove

spring and filter).

• Insert the ring diaphragm mount (2Ć21ā/ā1)

(contained in the delivery package of the

slider).

If required, loosen centering screws (2Ć21ā/ā2)

using the key.

• Insert ring diaphragm in ring diaphragm

mount and center the ring diaphragm with

regard to the phase ring of the objective

using the centering screws (one centering

screw at each side of the slider).

Figure 2Ć21Varā/āHā/āVar slider

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Zeiss Axiovert 135M Dokumentacja Strona 49