Zeiss Axiovert 135M Dokumentacja Pobierz pdf (Strona 50)

Axiovert 25, Axiovert 25 C, Axiovert 25 CFL

08/99 B 40Ć012 e

2Ć16

2.4.5 Reflected light fluorescence

requires the use of the Axiovert 25 CFL microscope stand.

• Select a point on the specimen in transmitted light brightfield or phase contrast. To do this, switch

the reflector mount (2Ć22ā/ā10) to free passage, switch on the halogen lamp (2Ć22ā/ā2) and move the

phase slider (2Ć22ā/ā3) to the middle position (brightfield) or to the position with the ring

diaphragm (phase contrast).

• Switch on the HBO 50 lamp (2Ć22ā/ā5) by means of the power supply unit, but block the light path

using the additional filter slider (2Ć22ā/ā6).

• After selecting the specimen position, cover the transmitted light beam path by means of the

cover plate (2Ć22ā/ā11) in the Phā/ā3Ćfold slider (2Ć22ā/ā3) or switch off the halogen lamp.

• With the reflector mount (2Ć22ā/ā10), slide in the required filter combination (2Ć22ā/ā9) and release

the light path by pulling the additional filter slide  (2Ć22ā/ā6).

• By means of the push rod (2Ć22ā/ā7), close the luminous field diaphragm until it is visible in the

image, move it to the middle position by means of the centring screws (2Ć22ā/ā8) and open it up

to the edge of the field of view.

• Additional excitation filters with a diameter of 25 mm, which must be held by means of zero

rings, can be inserted in the additional filter slider (2Ć22ā/ā6) of the reflected light unit Fl.

• The additional filter slider (2Ć22ā/ā6) allows no light to pass in the center stop position.

Figure 2Ć22 Observation under reflected light fluorescence

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Zeiss Axiovert 135M Dokumentacja Strona 50